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Image Search Results
Journal: bioRxiv
Article Title: PIN FORMED 2 facilitates the transport of Arsenite in Arabidopsis thaliana
doi: 10.1101/710160
Figure Lengend Snippet: Allocation of 74,73 As in Col-0 and eir1-1 (A) . Five-day-old Col-0 and eir1-1 roots were incubated in 0.1 μM and 10 μM 74,73 As for 2h. 74,73 As radiation was captured by an imaging plate (IP). Images are representative of three independent experiments. (B) Quantification of As allocation in root from experiment A . The data were obtained from three independent experiments with 10 seedlings in each treatment. Vertical bars mean ±SE. Asterisks represent the statistical significance between treatments as judged by Student’s t -test: **P < 0.001. (C) Scintillation counting 74,73 As activity in Col-0 and eir1-1 . Five-day-old Col-0 and eir1-1 seedlings were incubated for 2h at 0.1, 10 and 100 μM 74,73 As. Whole root was collected after the incubation. 74,73 As activity was measured by liquid scintillation counting. The data were obtained from 10 individual roots for each treatment. Vertical bars mean ±SD. Asterisks represent the statistical significance between treatments as judged by Student’s t -test: ***P < 0.0001. (D) Arsenic content in Col-0 and eir1-1 . Five-day-old light grown Col-0 and eir1-1 seedlings were transferred to 10 and 100 μM arsenite solution and incubated for 2h. 5 mm root tip of 20 seedlings for each treatment was used to measure As by ICP-MS. The data were obtained from three independent experiments. Vertical bars mean ±SE. Asterisks represent the statistical significance between treatments as judged by Student’s t -test: **P < 0.001 and ***P < 0.0001.
Article Snippet: Individual root sample was collected in a vial with scintillation cocktail (MICROSCINT 40, PerkinElmer) and 74,73 As activity was measured by liquid
Techniques: Incubation, Imaging, Activity Assay